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已发表论文
利用聚乙烯亚胺 - 海藻酸的纳米复合材料与骨形态发生蛋白 2 基因的复合物有效地改造细胞层，以促进新骨细胞形成
Authors Jin H, Zhang K, Qiao C, Yuan A, Li D, Zhao L, Shi C, Xu X, Ni S, Zheng C, Liu X, Yang B, Sun H
Published Date May 2014 Volume 2014:9(1) Pages 2179—2190
DOI http://dx.doi.org/10.2147/IJN.S60937
Received 17 January 2014, Accepted 15 March 2014, Published 7 May 2014
Abstract: Regeneration of large bone defects is a common clinical problem. Recently, stem cell sheet has been an emerging strategy in bone tissue engineering. To enhance the osteogenic potential of stem cell sheet, we fabricated bone morphogenetic protein 2 (BMP-2 ) gene-engineered cell sheet using a complex of polyethylenimine–alginate (PEI–al) nanocomposites plus human BMP-2 complementary(c)DNA plasmid, and studied its osteogenesis in vitro and in vivo. PEI–al nanocomposites carrying BMP-2 gene could efficiently transfect bone marrow mesenchymal stem cells. The cell sheet was made by culturing the cells in medium containing vitamin C for 10 days. Assays on the cell culture showed that the genetically engineered cells released the BMP-2 for at least 14 days. The expression of osteogenesis-related gene was increased, which demonstrated that released BMP-2 could effectively induce the cell sheet osteogenic differentiation in vitro. To further test the osteogenic potential of the cell sheet in vivo, enhanced green fluorescent protein or BMP-2-producing cell sheets were treated on the cranial bone defects. The results indicated that the BMP-2-producing cell sheet group was more efficient than other groups in promoting bone formation in the defect area. Our results suggested that PEI–al nanocomposites efficiently deliver the BMP-2 gene to bone marrow mesenchymal stem cells and that BMP-2 gene-engineered cell sheet is an effective way for promoting bone regeneration.
Keywords: bone regeneration, bone tissue engineering, nanocomposites, cell sheet, bone morphogenetic protein 2
Keywords: bone regeneration, bone tissue engineering, nanocomposites, cell sheet, bone morphogenetic protein 2
