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已发表论文
来自碲富集螺旋藻的含碲藻胆蛋白的纯化与体外抗氧化活性
Authors Yang F, Wong KH, Yang YF, Li XL, Jiang J, Zheng WJ, Wu HL, Chen TF
Published Date October 2014 Volume 2014:8 Pages 1789—1800
DOI http://dx.doi.org/10.2147/DDDT.S62530
Received 15 February 2014, Accepted 16 May 2014, Published 9 October 2014
Abstract: Tellurium-containing phycocyanin (Te-PC) and allophycocyanin (Te-APC), two organic tellurium (Te) species, were purified from tellurium-enriched Spirulina platensis by a fast protein liquid chromatographic method. It was found that the incorporation of Te into the peptides enhanced the antioxidant activities of both phycobiliproteins. With fractionation by ammonium sulfate precipitation and hydroxylapatite chromatography, Te-PC and Te-APC could be effectively separated with high purity, and Te concentrations were 611.1 and 625.3 µg g-1 protein in Te-PC and Te-APC, respectively. The subunits in the proteins were identified by using MALDI-TOF-TOF mass spectrometry. Te incorporation enhanced the antioxidant activities of both phycobiliproteins, as examined by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid assay. Moreover, Te-PC and Te-APC showed dose-dependent protection on erythrocytes against the water-soluble free radical initiator 2,2'-azo(2-asmidinopropane)dihydrochloride-induced hemolysis. In the hepatoprotective model, apoptotic cell death and nuclear condensation induced by tert-butyl hydroperoxide in HepG2 cells was significantly attenuated by Te-PC and Te-APC. Taken together, these results suggest that Te-PC and Te-APC are promising Te-containing proteins with application potential for treatment of diseases related to oxidative stress.
Keywords: tellurium, phycocyanin, allophycocyanin, purification, antioxidant activity
Keywords: tellurium, phycocyanin, allophycocyanin, purification, antioxidant activity
