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pEGFP - 装载芯-壳结构的脱乙酰壳多糖复合颗粒缓释行为及其在体内、体外的转染

 

Authors Wang Y, Lin FX, Zhao Y, Wang MZ, Ge XW, Gong ZX, Bao DD, Gu YF

Published Date October 2014 Volume 2014:9(1) Pages 4965—4978

DOI http://dx.doi.org/10.2147/IJN.S58104

Received 22 November 2013, Accepted 3 April 2014, Published 23 October 2014

Abstract: Novel submicron core-shell-structured chitosan-based composite particles ­encapsulated with enhanced green fluorescent protein plasmids (pEGFP ) were prepared by complex coacervation method. The core was pEGFP -loaded thiolated N-alkylated chitosan (TACS) and the shell was pH- and temperature-responsive hydroxybutyl chitosan (HBC). pEGFP -loaded TACS-HBC composite particles were spherical, and had a mean diameter of approximately 120 nm, as measured by transmission electron microscopy and particle size analyzer. pEGFP  showed sustained release in vitro for >15 days. Furthermore, in vitro transfection in human embryonic kidney 293T and human cervix epithelial cells, and in vivo transfection in mice skeletal muscle of loaded pEGFP , were investigated. Results showed that the expression of loaded pEGFP , both in vitro and in vivo, was slow but could be sustained over a long period. pEGFP  expression in mice skeletal muscle was sustained for >60 days. This work indicates that these submicron core-shell-structured chitosan-based composite particles could potentially be used as a gene vector for in vivo controlled gene transfection.
Keywords: gene therapy, gene transfection, hydroxybutyl chitosan, thiolated N-alkylated chitosan, pEGFP , complex coacervation






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