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Authors Yang B, Luo TH, Zhang MJ, Lu ZM, Xue XC, Fang GN
Received 7 February 2017
Accepted for publication 11 April 2017
Published 18 May 2017 Volume 2017:10 Pages 2635—2643
DOI https://doi.org/10.2147/OTT.S134121
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Colin Mak
Peer reviewer comments 2
Editor who approved publication: Dr Ingrid Espinoza
Abstract: Current evidence indicates that long noncoding RNAs (lncRNAs) play
pivotal roles in human cancers. The present study aims to assess differentially
expressed lncRNAs related to diffuse-type gastric carcinoma (DGC).
Next-generation RNA sequencing was carried out to detect aberrantly expressed
lncRNAs in DGC. Real-time polymerase chain reaction (RT-PCR) was performed to
evaluate RP11–357H14.17 gene expression levels in DGC cell lines/tissues
comparatively with normal gastric epithelial cell lines and adjacent normal
tissues. The associations of RP11–357H14.17 expression levels with the
clinicopathological features were also analyzed. The regulatory effects of
RP11–357H14.17 on the biological behaviors of DGC cells were evaluated by MTT,
colony formation assays, flow cytometry for apoptosis, wound healing assay, and
transwell migration and invasion assays. RP11–357H14.17 expression was
remarkably increased in DGC tissues and cell lines compared with normal gastric
epithelial cells and adjacent normal tissues. High levels of RP11–357H14.17
were associated with increased tumor size, deeper depth of invasion, lymphatic
metastasis, and advanced pathological stage. Further experiments demonstrated
that the DGC cells MGC-803 transfected with si-RP11–357H14.17 showed reduced
cell proliferation, migration, invasion, enhanced G1 phase arrest and cell
apoptosis. These findings suggest that the novel lncRNA RP11–357H14.17 is
associated with poor prognosis, and may serve as a potential prognostic
biomarker and target for new antineoplastic therapies in human DGC.
Keywords: long
noncoding RNA, RP11–357H14.17, diffuse-type gastric cancer, proliferation,
invasion, metastasis