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霍利迪 (Holliday) 接合识别蛋白质可促进细胞增殖并与肝细胞癌的不利临床结果相关

 

Authors Hu B, Wang Q, Wang Y, Chen J, Li P, Han M

Received 15 November 2016

Accepted for publication 7 January 2017

Published 17 May 2017 Volume 2017:10 Pages 2601—2607

DOI https://doi.org/10.2147/OTT.S127738

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Lucy Goodman

Peer reviewer comments 2

Editor who approved publication: Dr Samir Farghaly

Aim: To investigate the expression and clinical significance of Holliday junction–recognizing protein (HJURP) in hepatocellular carcinoma (HCC).
Methods: In this study, we detected the expression of HJURP protein in samples of 164 patients with HCC, and based on this, we divided the patients into two cohorts: high expression of HJURP and low expression of HJURP. We analyzed the correlation between HJURP expression and the clinicopathological factors using chi-square test. Survival significance of HJURP was defined by Kaplan–Meier method and log-rank test, and the independent prognostic factors were identified by Cox regression model. Using function assays of HCC cell lines, we investigated the influence of HJURP on the proliferation of HCC cells.
Results: In our study, the proportion of patients with high HJURP expression was 25.6%, which was significantly associated with the tumor size and Barcelona clinic liver cancer stage. Univariate analysis confirmed that high HJURP expression was remarkably associated with poorer overall survival rates (=0.003), as well as tumor number (=0.016), tumor differentiation (=0.047), TNM stage (=0.005), and Barcelona clinic liver cancer stage (=0.004). Multivariate analysis confirmed that high HJURP expression (<0.001) acted as an independent prognostic risk factor of unfavorable prognosis. Real-time polymerase chain reaction analysis revealed that the expression of HJURP was significantly higher in HCC tissues than that in the corresponding normal liver tissues. Moreover, we demonstrated that HJURP overexpression could accelerate HCC cell line proliferation, whereas HJURP knockdown could attenuate the proliferation.
Conclusion:
 High HJURP expression was an independent prognostic biomarker of HCC, predicting poorer prognosis. HJURP also played an important role in HCC cell proliferation.
Keywords: Holliday junction–recognizing protein, hepatocellular carcinoma, proliferation, prognosis