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Authors Hu B, Wang Q, Wang Y, Chen J, Li P, Han M
Received 15 November 2016
Accepted for publication 7 January 2017
Published 17 May 2017 Volume 2017:10 Pages 2601—2607
DOI https://doi.org/10.2147/OTT.S127738
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Lucy Goodman
Peer reviewer comments 2
Editor who approved publication: Dr Samir Farghaly
Aim: To investigate the expression and clinical significance of
Holliday junction–recognizing protein (HJURP) in hepatocellular carcinoma
(HCC).
Methods: In this study, we detected the expression of HJURP
protein in samples of 164 patients with HCC, and based on this, we divided the
patients into two cohorts: high expression of HJURP and low expression of
HJURP. We analyzed the correlation between HJURP expression and the
clinicopathological factors using chi-square test. Survival significance of
HJURP was defined by Kaplan–Meier method and log-rank test, and the independent
prognostic factors were identified by Cox regression model. Using function
assays of HCC cell lines, we investigated the influence of HJURP on the
proliferation of HCC cells.
Results: In our study, the proportion of patients with high
HJURP expression was 25.6%, which was significantly associated with the tumor
size and Barcelona clinic liver cancer stage. Univariate analysis confirmed
that high HJURP expression was remarkably associated with poorer overall
survival rates (P =0.003), as well as tumor number
(P =0.016), tumor differentiation (P =0.047), TNM stage (P =0.005), and Barcelona clinic
liver cancer stage (P =0.004).
Multivariate analysis confirmed that high HJURP expression (P <0.001) acted as an
independent prognostic risk factor of unfavorable prognosis. Real-time polymerase
chain reaction analysis revealed that the expression of HJURP was significantly
higher in HCC tissues than that in the corresponding normal liver tissues.
Moreover, we demonstrated that HJURP overexpression could accelerate HCC cell
line proliferation, whereas HJURP knockdown could attenuate the proliferation.
Conclusion: High HJURP expression was an
independent prognostic biomarker of HCC, predicting poorer prognosis. HJURP
also played an important role in HCC cell proliferation.
Keywords: Holliday junction–recognizing
protein, hepatocellular carcinoma, proliferation, prognosis